中國水產(chǎn)頻道獨家報道，2013年11月18日 美國消息，亞利桑那州--PCR快速檢測EMS/AHPND成為可能。

　　

　　亞利桑那大學(xué)對蝦病理實驗室Dr. Donald Lightner團隊的助理研究員Linda Nunan給對蝦養(yǎng)殖從業(yè)者發(fā)布了以下信息：

　　

　　親愛的對蝦養(yǎng)殖業(yè)同仁：

　　

　　我代表亞利桑那大學(xué)技術(shù)轉(zhuǎn)讓辦公室發(fā)布以下聲明：當(dāng)前可應(yīng)用PCR技術(shù)檢測AHPND。

　　

　　急性肝胰臟壞死病（AHPND）引起中國、越南、泰國和馬來西亞養(yǎng)殖對蝦大量死亡。自2009年該病被首次報道以來，該疾病造成損失超過10億美元/年。亞利桑那大學(xué)研究人員已經(jīng)發(fā)現(xiàn)AHPND由定植于對蝦腸道的細菌所引起，其可對宿主肝胰腺等消化器官造成組織破壞。研究人員發(fā)現(xiàn)AHPNS病原體為常見海洋細菌——副溶血性弧菌的一種特殊變異菌株。該菌株具有一個1.8萬對的堿基序列，且僅在病原分離物中發(fā)現(xiàn)，故其可作為PCR檢測AHPND的目標基因序列。

　　

　　當(dāng)前，應(yīng)用組織學(xué)鑒定是判斷對蝦是否感染AHPND的唯一方法，該方法耗時長，費用高。應(yīng)用PCR技術(shù)開展快速病原檢測技術(shù)將迅速投入市場，促進對蝦產(chǎn)業(yè)發(fā)展。該目標序列具有獨特性，在NCBI數(shù)據(jù)庫中不存在同源匹配序列。伴隨進口對蝦比例占美國總消費量比如攀升至85％以上，快速檢測AHPND技術(shù)對海鮮市場將有著極高價值。

　　

　　技術(shù)優(yōu)勢：

　　

　　實現(xiàn)AHPND的快速檢測；

　　

　　檢測成本低，且無需組織學(xué)分析；

　　

　　大批量檢測成為可能。

　　

　　November 18, 2013

　　

　　United States

　　

　　Arizona—PCR Detection of Acute Hepatopancreatic Necrosis Disease (AHPND/EMS) Available!!!

　　

　　Linda Nunan, Assistant Staff Scientist at Dr. Donald Lightner’s shrimp pathology laboratory at the University of Arizona, posted the following information to The Shrimp List, a mailing list for the shrimp farming industry:

　　

　　“Dear shrimp list people:

　　

　　I am posting the following for the Office of Technology Transfer at the University of Arizona.  The technology for PCR detection of AHPND is now available.

　　

　　Acute Hepatopancreatic Necrosis Disease (AHPND) is causing high mortalities in farm-raised shrimp cultured in China, Vietnam, Thailand and Malaysia.  Since the disease was first reported in 2009, it now causes annual losses of more than $1 billion.  Faculty at the University of Arizona have found that AHPND is caused by a bacterial agent which colonizes the intestinal tract of shrimp and causes tissue destruction of the shrimp digestive organ known as the hepatopancreas.  The inventors found the AHPNS pathogen is a unique strain of the common marine bacterium Vibrio parahaemolyticus.  The unique strain possess an 18 kilo-base pair gene sequence that is only present in the pathogenic isolate, so it can be a target for detection of AHPND using a PCR assay.

　　

　　Currently, the only method to determine if a shrimp is infected with AHPND causing bacterium is through the use of histology, which is time consuming and expensive.  A rapid PCR test for the detection of this pathogen will be the first on the market and will be beneficial to the shrimp producing industry.  The targeted sequence is also unique; there are no homologous matches to any other sequences in the NCBI database.  With imports of shrimp into USA upwards of 85% of total consumption, quick detection of AHPND could be very valuable to the seafood market.

　　

　　Advantages

　　

　　• Rapid detection of AHPND

　　

　　• Cost effective without the need for histology

　　

　　• Able to test much larger quantities of shrimp”