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標題: 求大神翻譯 [打印本頁]

作者: 上帝的愛 時間: 2015-12-12 11:58
標題: 求大神翻譯
A microsporidian parasite, Enterocytozoon hepatopenaei (abbreviated as EHP), is an emerging pathogen for penaeid shrimp. EHP has been found in several shrimp farming countries in Asia including Vietnam, Thailand, Malaysia, Indonesia and China, and is reported to be associated with growth retardation in farmed shrimp. We examined the histological features from infected shrimp collected from Vietnam and Brunei, these include the presence of basophilic inclusions in the hepatopancreas tubule epithelial cells, in which EHP is found at various developmental stages, ranging from plasmodia to mature spores. By a PCR targeting the 18S rRNA gene, a 1.1聽kb 18S rRNA gene fragment of EHP was amplified, and this sequence showed a 100% identity to EHP found in Thailand and China. This fragment was cloned and labeled with digoxigenin-11-dUTP, and in situ hybridized to tissue sections of infected Penaeus vannamei (from Vietnam) and P. stylirostris (Brunei). The results of in situ hybridization were specific, the probe only reacted to the EHP within the cytoplasmic inclusions, not to a Pleistophora -like microsporidium that is associated with cotton shrimp disease. Subsequently, we developed a PCR assay from this 18S rRNA gene region, this PCR is shown to be specific to EHP, did not react to 2 other parasitic pathogens, an amoeba and the cotton shrimp disease microsporidium, nor to genomic DNA of various crustaceans including polychaetes, squids, crabs and krill. EHP was detected, through PCR, in hepatopancreatic tissue, feces and water sampled from infected shrimp tanks, and in some samples of Artemia biomass.

作者: 上帝的愛 時間: 2015-12-12 12:00
一個個拆開我還可以讀出來，有關孢子蟲的，大家幫組一下，目前正在研究這個問題

作者: 狼牙166375 時間: 2015-12-14 10:46
一種微孢子寄生蟲-對蝦腸孢子蟲（縮寫為EHP），是一種針對對蝦的新興病原體，目前已在亞洲包括越南、泰國、馬來西亞、印度尼西亞和中國的幾個蝦養殖國家發現，報道稱該種病原能導致蝦生長遲緩。我們檢查了收集自越南和文萊感染蝦體的組織特征，這其中就包含了嗜堿性夾雜物的肝曲小管上皮細胞，在這里發現了EHP從瘧原蟲到成熟孢子的多個發育階段，通過PCR特異性擴增該EHP的18SrRNA基因，一條長為1.1kbp的EHP18SrRNA基因片段被擴增出來，且該片段的基因序列與在泰國和中國發現的EHP序列顯示出100%的同源性。該基因片段被克隆后被標上地高辛-11-dUTP標記，然后原位雜交到南美白對蝦（來自越南）和南美白細腳對蝦（文萊）的感染組織部分，原位雜交的結果是特異性的，探針只在EHP的細胞質內含物中發生了反應，而不是一種與棉花蝦疾病相關的具褶孢蟲屬的孢子蟲。隨后，我們又針對這個EHP的18SrRNA基因區域進行了PCR檢測，其結果顯示對EHP是特異性的,并不與其他兩種孢子蟲病原發生反應，阿米巴和棉花蝦疾病微孢子蟲，也不是其他甲殼類包括多毛類、魷魚、螃蟹和磷蝦的基因組DNA，通過PCR我們在感染蝦塘的蝦肝臟組織、糞便、水樣和一些鹵蟲的樣品中發現了EHP。

作者: 一片云 時間: 2015-12-15 12:06

狼牙166375 發表于 2015-12-14 10:46
一種微孢子寄生蟲-對蝦腸孢子蟲（縮寫為EHP），是一種針對對蝦的新興病原體，目前已在亞洲包括越南、泰國、 ...

給力啊！給你點贊

作者: 上帝的愛 時間: 2015-12-17 23:49

狼牙166375 發表于 2015-12-14 10:46
一種微孢子寄生蟲-對蝦腸孢子蟲（縮寫為EHP），是一種針對對蝦的新興病原體，目前已在亞洲包括越南、泰國、 ...

謝謝狼牙老板，這個月開始系統研究和處理對蝦親蝦防控腸孢子蟲

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